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Table 3 Quantitative analysis of A. marginale colony densities in D. variabilis guts and salivary glands after gene knockdown by RNAi.

From: Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

 

Tick genes silenced by RNAi

Tissue/colonies containing RF or DF

GST

SelM

vATPase

Subolesin

Control

Ticks collected after AF

     

Gut/RF

0.27 ± 0.24

0.85 ± 0.31*

0.62 ± 0.57

0.00 ± 0.00*

0.28 ± 0.20

Gut/DF

0.07 ± 0.01*

0.26 ± 0.26

0.15 ± 0.12

0.17 ± 0.06

0.18 ± 0.13

Salivary glands/RF

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

Salivary glands/DF

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

Ticks collected after TF

     

Gut/RF

1.00 ± 0.72

1.43 ± 1.29*

0.63 ± 0.47

0.04 ± 0.01*

0.75 ± 0.59

Gut/DF

0.29 ± 0.23

0.53 ± 0.48

2.62 ± 2.31*

0.04 ± 0.03*

0.32 ± 0.25

Salivary glands/RF

0.00 ± 0.00*

0.00 ± 0.00*

0.00 ± 0.00*

0.00 ± 0.00*

0.003 ± 0.001

Salivary glands/DF

0.00 ± 0.00*

0.00 ± 0.00*

0.00 ± 0.00*

0.00 ± 0.00*

0.01 ± 0.01

  1. The density of A. marginale reticulated forms (RF) and dense forms (DF) containing colonies (average ± SD) was calculated for tick gut and salivary gland (sg) sections after acquisition feeding (AF) and transmission feeding (TF) and compared between dsRNA-injected and control ticks by Student's t-test with unequal variance (*P < 0.05). The values were underlined when gene knockdown resulted in higher RF or DF when compared to controls.