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Table 3 Quantitative analysis of A. marginale colony densities in D. variabilis guts and salivary glands after gene knockdown by RNAi.

From: Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

  Tick genes silenced by RNAi
Tissue/colonies containing RF or DF GST SelM vATPase Subolesin Control
Ticks collected after AF      
Gut/RF 0.27 ± 0.24 0.85 ± 0.31* 0.62 ± 0.57 0.00 ± 0.00* 0.28 ± 0.20
Gut/DF 0.07 ± 0.01* 0.26 ± 0.26 0.15 ± 0.12 0.17 ± 0.06 0.18 ± 0.13
Salivary glands/RF 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Salivary glands/DF 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Ticks collected after TF      
Gut/RF 1.00 ± 0.72 1.43 ± 1.29* 0.63 ± 0.47 0.04 ± 0.01* 0.75 ± 0.59
Gut/DF 0.29 ± 0.23 0.53 ± 0.48 2.62 ± 2.31* 0.04 ± 0.03* 0.32 ± 0.25
Salivary glands/RF 0.00 ± 0.00* 0.00 ± 0.00* 0.00 ± 0.00* 0.00 ± 0.00* 0.003 ± 0.001
Salivary glands/DF 0.00 ± 0.00* 0.00 ± 0.00* 0.00 ± 0.00* 0.00 ± 0.00* 0.01 ± 0.01
  1. The density of A. marginale reticulated forms (RF) and dense forms (DF) containing colonies (average ± SD) was calculated for tick gut and salivary gland (sg) sections after acquisition feeding (AF) and transmission feeding (TF) and compared between dsRNA-injected and control ticks by Student's t-test with unequal variance (*P < 0.05). The values were underlined when gene knockdown resulted in higher RF or DF when compared to controls.