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Table 2 A. marginale infection levels in D. variabilis male guts and salivary glands after RNAi of selected tick genes.

From: Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

Experimental group

Infection levels in guts after AF (A. marginale/tick ± SE)

Infection levels in guts after TF (A. marginale/tick ± SE)

Infection levels in salivary glands after TF (A. marginale/tick ± SE)

GST

5 ± 15*

99,060 ± 68462

2 ± 0*

vATPase

81 ± 5*

795 ± 227

247 ± 205

SelM

389,095 ± 282048

1,451 ± 443

2 ± 0*

Subolesin

814 ± 122

1,517 ± 1025

2 ± 0*

Saline control

40,579 ± 6993

28,252 ± 27788

287 ± 144

  1. The A. marginale infection levels were analyzed in D. variabilis male ticks after RNAi. Salivary glands and/or guts were dissected from 5 ticks after AF and TF and DNA was used for quantitative msp4 PCR to determine A. marginale infection levels. Infection levels in tick guts and salivary glands were expressed as average ± SE and compared between dsRNA and saline injected ticks by Student's t-test (*P ≤ 0.05).
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BMC Developmental Biology

ISSN: 1471-213X

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