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Table 1 Expression silencing of selected genes in D. variabilis male guts and salivary glands after RNAi.

From: Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

Experimental group Silencing in guts after AF (% ± SD) Silencing in guts after TF (% ± SD) Silencing in salivary glands after TF (% ± SD)
GST 81.2.6 ± 12.4* 100 ± 0.0* 100 ± 0.4*
vATPase ND ND ND
SelM 74.1 ± 17.3* 100 ± 0.0* 74.0 ± 25.2*
Subolesin 90.0 ± 21.4* 99.7 ± 0.6* 99.4 ± 0.0*
  1. The silencing of gene expression was analyzed in D. variabilis male ticks after RNAi. Salivary glands and/or guts were dissected from 5 ticks after AF and TF and mRNA levels were analyzed by real time RT-PCR. Percent reduction in transcript levels relative to that in tissues from control ticks were averaged over five replicate samples and expressed as average ± SD. Significance was determined by comparing mRNA levels between dsRNA and saline injected control ticks by Student's t-test (*P ≤ 0.05). Abbreviation: ND, not done because RT-PCR conditions could not be established.