Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

Table 1 Expression silencing of selected genes in D. variabilis male guts and salivary glands after RNAi.

Experimental group	Silencing in guts after AF (% ± SD)	Silencing in guts after TF (% ± SD)	Silencing in salivary glands after TF (% ± SD)
GST	81.2.6 ± 12.4*	100 ± 0.0*	100 ± 0.4*
vATPase	ND	ND	ND
SelM	74.1 ± 17.3*	100 ± 0.0*	74.0 ± 25.2*
Subolesin	90.0 ± 21.4*	99.7 ± 0.6*	99.4 ± 0.0*

The silencing of gene expression was analyzed in D. variabilis male ticks after RNAi. Salivary glands and/or guts were dissected from 5 ticks after AF and TF and mRNA levels were analyzed by real time RT-PCR. Percent reduction in transcript levels relative to that in tissues from control ticks were averaged over five replicate samples and expressed as average ± SD. Significance was determined by comparing mRNA levels between dsRNA and saline injected control ticks by Student's t-test (*P ≤ 0.05). Abbreviation: ND, not done because RT-PCR conditions could not be established.

ISSN: 1471-213X