Figure 6

Semithin sections and whole mounts of 48 hour tail plate blastemas. Schemes are indicating the orientation of the animal in the respective subpanels. (A) At the anterior end (left), the blastema is delimited by the gut. Note the mitoses (arrowheads) within the blastema. An early duo-gland adhesive system, with a cell body close to the epidermis, has been regenerated (double arrowhead). Arrow points at a rhabdite gland. Stained after Heidenhain. (B, C) BrdU pulse. Accumulation of labeled cells in the blastema in whole mount (B) and section (C). Asterisk marks a label-free spot, the presumptive point of origin of the male genital apparatus. (B, C) from the same animal. (D, E) BrdU pulse-chase. (D) Labeled cells are concentrated in the blastema, and also appear in the epidermis (arrowhead). (E) Section of a different animal. Arrowheads in (D, E) point at labeled epidermal cells. Dotted lines indicate blastema extension. (C, E) stained with methylene blue. Scale bars are 20 μm.