Figure 2
The tail plate of M. lignano. (A-C) Electron micrograph of an adult specimen fixed with high pressure freezing. (A) Cross section through the tail plate at the tip of the stylet with representative cell types. Dorsal side is up. (B, C) Details of a more anterior tail plate section than (A). (B) The stylet is surrounded by musculature. The inner epithelial lining is interspersed by muscle cells as well. (C) The tip of a duo-gland adhesive system besides a new epidermis cell in differentiation. ci cilia dg duo-gland adhesive systems, dm dorsoventral muscles, ep multiciliated epidermal cell, gl cyanophil gland cell, mu muscle fibers, ne neoblast, np new epidermal cell, nv nerve, pg prostatic gland, rh rhabdite gland, rm ring muscles, st stylet. (D-G) Fluorescent whole mount stainings of the tail plate. (D) Neoblasts in S-phase labeled with BrdU, (E) epidermal nuclei (inset shows a single lobulated epidermal nucleus) labeled with Hoechst 33342, (F) muscle F-actin stained with phalloidin, (G) GYIRF-amidergic nervous system. Scale bar in (A) is 5 μm, in (B, C) 1 μm, in (D-G) 100 μm.