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Table 2 Accumulation of genomic material in nuclei of cap-gs105 mutants, cap-h, and cap-d2 morphants.

From: Non-SMC condensin I complex proteins control chromosome segregation and survival of proliferating cells in the zebrafish neural retina

 

DNA content fraction

 

<2C

2C

2C – 4C

4C

>4C

WT

2.2% ± 0.3%

87.9% ± 0.1%

4.5% ± 0.5%

3.5% ± 0.4%

1.3% ± 0.1%

cap-gs105

6.8% ± 1.7%

65.8% ± 4.0%

6.1% ± 0.5%

15.5% ± 1.3%

4.2% ± 0.7%

MOcap-h

4.3% ± 1.9%

70.1% ± 3.7%

8.2% ± 0.8%

13.1% ± 0.3%

2.6% ± 1.0%

MOcap-h+p53

3.7% ± 1.2%

74.2% ± 8.7%

6.6% ± 2.1%

11.2% ± 3.8%

3.0% ± 1.2%

MOcap-d2

9.8% ± 5.4%

61.4% ± 10.7%

11.3% ± 3.0%

10.0% ± 1.5%

4.7% ± 1.5%

MOcap-d2+p53

7.6% ± 3.5%

64.4% ± 14.2%

9.3% ± 3.2%

12.2% ± 4.2%

4.8% ± 2.9%

 

WT vs. cap-gs105

 

p < 0.05

p < 0.01

p < 0.01

p < 0.001

p < 0.01

 

WT vs. MOcap-h

 

p > 0.05

p < 0.01

p < 0.01

p < 0.001

p > 0.05

 

WT vs. MOcap-d2

t-test

p-values

     
 

p > 0.05

p < 0.05

p < 0.05

p < 0.005

p < 0.05

 

MOcap-hvs. MOcap-h+p53

 

p > 0.05

p > 0.05

p > 0.05

p > 0.05

p > 0.05

 

MOcap-d2 vs. MOcap-d2+p53

 

p > 0.05

p > 0.05

p > 0.05

p > 0.05

p > 0.05

  1. Table showing the distribution of nuclear DNA contents among all counted cells. Values represent an average of four independent experiments. t-test p-values (lower table) reveal a significant shift from diploid to aneuploid and especially tetraploid nuclei in cap-gs105, cap-h morphants or cap-d2 morphants compared to wild-type. There are no significant differences in the genomic content distributions of cap-h morphant and cap-h/p53 double morphant or of cap-d2 morphant and cap-d2/p53 double morphant retinae.