Figure 5
From: The pluripotency factor LIN28 marks undifferentiated spermatogonia in mouse
Ngn3 -GFP labels a more committed subpopulation of LIN28-positive spermatogonia. Seminiferous tubules from adult Ngn3-GFP mice were immunostained with anti-LIN28 and anti-GFP antibodies [26]. We used antibodies to visualize GFP, since the GFP fluorescence was weak. (A) LIN28-positive undifferentiated spermatogonia are divided into Ngn3-GFP-positive and Ngn3-GFP-negative subpopulations. As, Apr and the number of Aal spermatogonia were indicated. Ngn3-GFP-negative spermatogonia were circled. Scale bar, 25 μm. (B) Frequency of spermatogonia clusters (2n cells: 1, 2, 4, 8, 16) with cells that are either all Ngn3-GFP-positive or all Ngn3-GFP-negative. The total number of 2n-cell clusters examined was shown above each column.