Figure 4
From: Red fluorescent Xenopus laevis: a new tool for grafting analysis
Tissue identity of blastema derived ectopic tails. 21 days old ectopic tails or control regenerates (C,F,I) were isolated and immunostained with specific antibodies directed against muscle cells (12/101)(A-C), neuronal cells (Xen-1)(D-F) or notochord (anti-Coll-2)(G-I). Scale bars equal 100 μm in A, D, G or 1 mm in C, F, I. Note, that the red fluorescence reflects the Cy3 labelled secondary antibody exclusively, as the red fluorescence of the tdTomato protein is destroyed by the fixation used for immunostaining.