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Figure 4 | BMC Developmental Biology

Figure 4

From: Red fluorescent Xenopus laevis: a new tool for grafting analysis

Figure 4

Tissue identity of blastema derived ectopic tails. 21 days old ectopic tails or control regenerates (C,F,I) were isolated and immunostained with specific antibodies directed against muscle cells (12/101)(A-C), neuronal cells (Xen-1)(D-F) or notochord (anti-Coll-2)(G-I). Scale bars equal 100 μm in A, D, G or 1 mm in C, F, I. Note, that the red fluorescence reflects the Cy3 labelled secondary antibody exclusively, as the red fluorescence of the tdTomato protein is destroyed by the fixation used for immunostaining.

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