The DEAD-box protein MEL-46 is required in the germ line of the nematode Caenorhabditis elegans

Table 3 Temperature dependent decrease in brood size of mel-46(yt5) mutants

Temperature	Genotype^a	n^b	Mean	Std Dev	p^c
15°C	+/+ or mel-46(yt5)/ +	12	306.5	20.8	0.492
	mel-46(yt5)	13	294.6	36.2
20°C	+/+ or mel-46(yt5)/ +	20	309.4	29.7	0.743
	mel-46(yt5)	10	305.2	54.5
23°C	+/+ or mel-46(yt5)/ +	14	277.6	41.3	0.00644
	mel-46(yt5)	14	210.2	66.7
25°C	+/+ or mel-46(yt5)/ +	20	185.0	23.7	1.99^e-10
	mel-46(yt5)	17	45.4	32.9

^a All animals were the progeny of mel-46(yt5) / + mothers. Animals that produced predominantly (at lower temperatures) or exclusively dying embryos were considered to be mel-46(yt5) homozygous. ^b Only animals that were fertile were included in this analysis. ^c two tailed paired t-test between the genotypes within the temperature.

ISSN: 1471-213X