Figure 11

C/EBPα protein immunolocalization in WT and Tabby glands in vivo and in cultured SMGs. A, B. In vivo distribution of C/EBPα protein in E17 WT (A) and Tabby (B) SMGs. C/EBPα protein is primarily localized in nuclei (arrowheads) of epithelial cells surrounding ductal and terminal bud lumina in E17 SMGs. There is a notable decrease in nuclear-localized C/EBPα protein, as well as a marked increase in DAPI-stained nuclei, in Tabby glands (B) compared to WT glands (A). C, D. C/EBPα protein immunolocalization in cultured control (C) and Eda-A1-treated (D) explants. Eda treatment (D) induces a marked increase in immunodectable and nuclear-localized (arrowheads) C/EBPα protein compared to controls (compare D to C). Note that in controls glands (C), C/EBPα protein is found in the cytoplasm whereas the nuclei are labeled with DAPI alone (arrows). Inserts C, D. Higher magnifications showing cytoplasmic-localized C/EBPα protein in controls (C) and nuclear-localized C/EBPα protein in Eda-treated (D) SMGs. Bar, A-B: 20 μm, C-D: 30 μm; C-D inserts: 10 μm.