Figure 2

Otic markers and vesicle morphology are unaffected at E10.5 in TCre-KO mutants. Expression of sensory (A-F) and neurogenic (G-J) markers are unchanged in TCre-KO embryos compared to controls at E10.5. Ventral markers Pax2 and Otx1, as well as dorsal markers Bmp4 and Dlx5 are normal in TCre-KO mutants (A'-D'). Gata3 expression in the lateral otic vesicle is also unchanged in the TCre-KO, visualized in cross sections through the otic vesicle (F'). Expression of anterior markers NeuroD, Fgf3, Lnfg and Fgf10 display normal expression in the CVG in both mutants (G'-J') and controls (G-J). Expression of Lmx1a, which marks the growing endolymphatic duct, is also unchanged (K, K'). Microdissected otic vesicles are shown at E10.5 (L, L') and E11.5 (M, M'). At E10.5, the morphology of the otic vesicle in control (L) and TCre-KO (L') embryos is similar. By E11.5, the otic vesicles of TCre-KO embryos (M') are malformed and hypoplastic compared to controls (M). Scale bar in L is 250 μm and applies to all panels except (E, E') and (F, F'). Scale bars in E' and F' are 200 μm.