Figure 8

Apoptosis during radial nerve cord regeneration. (A.) Longitudinal section of regenerating RNC at 6 dpi showing TUNEL labeling (green) in cells undergoing apoptosis (arrows) (counter-stained with Hoescht-red). Apoptosis occurs in both (B) hyponeural and (C) ectoneural components of the regenerating radial nerve cord peaking at day 6 dpi in the proximal nerve stumps and at 12 dpi in the regenerating cord that is forming at the injury site. Little or no apoptosis occurs in areas distal to the injury. Regenerated tissues in the injured area do not appear until 12-dpe. Each point represents the mean ± S.E. of five animals. When compared to distal, significant differences are observed at various stages in the proximal and injured areas *p < .05, **p < .01. HN – hyponeural component, EN – ectoneural component. Bar = 25 μm.