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Figure 2 | BMC Developmental Biology

Figure 2

From: Regeneration of the radial nerve cord in the sea cucumber Holothuria glaberrima

Figure 2

RN1 labeling of the regenerating radial nerve cord. Longitudinal tissue sections of (A) uninjured and regenerating radial nerve cords at (B) 2, (C) 6, (D) 12, (E) 20 and (F) 28 days post injury (dpi) were labeled with the monoclonal antibody RN1. (A) Labeling in the uninjured RNC is highly specific to the nervous components, both ectoneural (EN) and hyponeural (HN) component while the connective tissue band remains unlabeled (arrowhead). (B) At 2 dpi nerve fiber debris is visible around the injury site and the nerve stumps can be seen to be highly disorganized. (C) By 6 dpi, the RNC stumps have organized into club-shaped structures from which some nerve fibers can be observed to be extending (arrows). (D) By 12 dpi the injury gap has been filled with nervous tissue that now forms a continuous extension that joins the stumps. (E) By 20 dpi the RNC still appears slightly disorganized and the CT band is not present along the entire length of the regenerated RNC. (F) At 28 dpi, the RNC has recovered much of the structure and organization found within non-injured RNC including the connective tissue that separates the EN and HN components. EN-epineural component, HN-hyponeural component, RNC-radial nerve cord. X's denote the injury site; Bar = 300 μm.

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