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Table 1 Summary of cDNA fragments identified by the DD-PCR screen of ventral and dorsal midbrain regions.

From: Identification of genes differentially expressed in dorsal and ventral chick midbrain during early Development

Clone ID

Gene Bank

accession number

Gene product

Function

Expression

M5v3

XM_001236352.1

Kinase (PRKA) anchor protein 8-like (AKAP8L)

Cell division

n.d.

M12v2

NM_001031296.1

Splicing factor, arginine/serine-rich 11 SFRS11

RNA processing

n.d.

F1d1

NW_001471710

SRP14

RNA processing

Verified by RT-PCR

F0d1a

XM_414248.2

Nucleolar RNA host

gene 8

Nucleolar RNA

n.d

F0d4

XM_001234485

SMARCA5

Chromatin modification

n.d.

M5v1

NW_001471669

Estrogen receptor α

Transcription

Dorso-ventral gradient

M0d1a

ref/NC_026897.1

16S ribosomal RNA

RNA metabolism

n.d.

M6v2

XM_414131

CCDC123

Mitochondrial protein

n.d.

M17d2

XM_415003.2

Mitochondrial ribosomal protein S5

Mitochondrial protein

n.d.

M4v1

NM_001030819

ABCC4 (CFTR/MRP)

Chloride channel

n.d.

M5v4, M12v3

NW_001471710

MEKKK5

Signalling

Dorso-ventral gradient

M7v1

NW_001471526

AAT1-α

Signalling

Dorso-ventral gradient

M13v1a

XR_026877.1

Sulfatase 1 SULF 1

Signalling

n.d.

M4d2

NW_001471434

Naked cuticle (NKD1) homologue 1

Signalling

Verified by RT-PCR

F0d3

NW_001471529

Similar to EAT2

Signalling

Verified by RT-PCR

F4d1

emb/AJ720092

YWHAH

Signalling

n.d.

M5v2

XM_419938

KIAA0161, ring finger protein 144A (RNF144a)

Protein turnover

n.d.

M11d1

NW_001471685

QR1 (SPARC-like 1, Hevin)

Cell adhesion

Medio-dorsal

M5v6

NW_001471633

TEM7R/Plxdc2

Migration

Verified by RT-PCR

F0d1, F0d2

NW_001471503

Tenascin C

ECM

Verified by RT-PCR

M1v1

XM_426638

Ferric-chelate reductase 1

Iron metabolism

n.d.

M12d2

NM_001012604.

Macrophage erythroblast attacher MAEA

Migration

n.d

M1v1a

ref/XR_026897.1

Similar to MLL5

Chromatin modifications

n.d.

F4v1

XM_415718.2

RNF43

Protein turnover

n.d.

  1. The table itemizes the BLAST accession numbers, the chick nomenclature, the function and expression of the identified clones. The code of the clone IDs is as follows: M and F followed by the number stand for the primer combination used to make cDNA, v = ventral, d = dorsal. Abbreviations used: ECM = extracellular matrix, n.d. = not determined.