Figure 5From: Adenomatous polyposis coli-mediated control of β-catenin is essential for both chondrogenic and osteogenic differentiation of skeletal precursorsAbnormal axial skeleton formation of Col2a1- Cre ; Apc15lox/15lox embryos already detectable at E12.5. (A) Immunostaining for β-catenin combined with Alcian blue (AB) staining, and (B-E) gene expression analysis by in situ hybridization with indicated probes on consecutive transversal sections of the sclerotome of a Col2a1-Cre;Apc15lox/15lox embryo and control littermate at E12.5. (F-J) Similar analysis of vertebrae primordia at E14.5. β-Catenin-positive spindle-shaped cells lacked expression of all indicated chondrogenic and osteogenic markers (arrowheads). Scale bars: 100 μm.Back to article page