Figure 4

Details of skeletal preparations. (A-D) Vertebral column of control and mutant littermates at E14.5 (A, B) and E16.5 (C, D). Mutant vertebrae lacked structural integrity (arrowheads). At E16.5, mineralization was enhanced in the proximal part of the mutant rib (arrow). (E-H) Forelimb of control and mutant littermates at E14.5 (E, F) and E16.5 (G, H). At E16.5, only the scapula was identified (arrowhead), while more distal parts were represented by patchy cartilage aggregations (arrow). (I, J) Skull of control and mutant littermates at E16.5. The mutant displayed mineral deposition in the back skull corresponding to the cartilaginous structure in the control (arrowheads). Scale bars: 3 mm in A-H; 1 mm in I, J.