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Figure 5 | BMC Developmental Biology

Figure 5

From: Spatiotemporal association of DNAJB13 with the annulus during mouse sperm flagellum development

Figure 5

In vitro studies of DNAJB13 and SEPT4. (A) GFP-DNAJB13 (green, upper panel) was co-localized with FLAG-SEPT4 (red) in HeLa S3 cells. The cell nuclei were stained with DAPI. The scale bar represents 10 μm. (B) HeLa S3 cells were co-tranfected with plasmids pGFP-Dnajb13 and p3xFLAG-Sept4. Twenty-four hours later the cells were lysed and subjected to Co-IP with normal rabbit IgG or rabbit anti-GFP antibody. The lysate (input) together with the Co-IP products were then subjected to immunoblotting with mouse anti-FLAG antibody and mouse anti-GFP antibody respectively. IB: immunoblotting.

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