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Figure 4 | BMC Developmental Biology

Figure 4

From: Activation of an AMP-activated protein kinase is involved in post-diapause development of Artemia franciscana encysted embryos

Figure 4

Cellular ATP, ADP, and AMP were determined by HPLC analysis. A. HPLC chromatogram of ATP, ADP, and AMP from Artemia diapause embryos (a) and embryos at three representative developing stages (0, 4, and 8 h, b-d). Arrows indicate peaks of ATP, ADP, and AMP. B. Concentrations of cellular ATP, ADP, and AMP in embryos at different developmental stages were determined by an external standard method. dp, diapause embryos. C. Changes of ADP:ATP (white columns) and AMP:ATP (black columns) ratios in developing embryos.

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