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Figure 3 | BMC Developmental Biology

Figure 3

From: Activation of an AMP-activated protein kinase is involved in post-diapause development of Artemia franciscana encysted embryos

Figure 3

Subcellular location of activated AMPK. A. Supernatant (S) and pellet (P) fractions were prepared using buffer K (pH 6.0 or 8.0) from 4-h incubated embryos and separated by 10% SDS-PAGE. Proteins were also transferred to PVDF membranes and detected using phospho-AMPK (Thr172) antibody. The detected AMPK is indicated by an arrow. B. Confocal microscopy confirms that AMPK localizes in the nuclei when activated. Nuclei were double-stained using DAPI (a) and FITC-conjugated goat anti-rabbit IgG (b) after incubation with phospho-AMPK (Thr172) antibody. c, merged image of a and b. d, d' low magnification image of a field of nuclei double-stained with FITC-conjugated goat anti-rabbit IgG (d) and DAPI (d').

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