Figure 1

Activation of AMPK during developmental stages of Artemia. A. phosphorylated AMPK was detected using phospho-AMPK (Thr172) antibody by immunoblotting extracts from diapause embryos, developing post-diapause embryos (0 – 12 h), nauplii, and adults. α-Tubulin was used as a loading control. B. In vitro kinase assay to test AMPK activity from PEG-precipitated proteins. GST-SAMS or GST from the reaction buffer were separated by 15% SDS-PAGE and phosphorylated proteins were detected by Western blotting using Biotinylated Phos-tag™. Coomassie staining of GST proteins as a loading control is shown in the lower panel. The presence of phosphorylated AMPK in the precipitates was determined by Western blotting with phospho-AMPK antibody. p-AMPK, phosphorylated AMPK; p-GST-SAMS, phosphorylated GST-SAMS.