Figure 5From: Rac1 regulates pancreatic islet morphogenesisBlocking Rac1 activation inhibits plating of islets in vitro. (A,B) Isolated islets were seeded onto gelatin coated tissue culture dishes and incubated for one week. Whereas wild-type islets plate out and show actin remodelling; marked by phalloidin staining of stress fibres (arrows) of leading-edge cells (C), the RIP-RacN17 islets remained intact (B) with minor actin remodelling (D). (E) Quantification of the plating-efficiency of islets after one week in culture showed lower plating-efficiency of transgenic islets compared to wild-type islets (n = 5). Arrowheads in C and D indicate focal contacts and junctional F-actin, respectively. Error bars represent standard deviation from the mean (± s.d.) and significant values are indicated as *p < 0.05 determined by Student's t-test. Bars, 100 μm (A,B) and 20 μm (C,D).Back to article page