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Figure 3 | BMC Developmental Biology

Figure 3

From: A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera

Figure 3

Recombinant expression of Ha-eIF5C in E. coli and the preparation of anti-Ha-eIF5C antiserum. A: SDS-PAGE analysis of recombinantly expressed Ha-eIF5C in E. coli. Lane 1, non-induction control; lane 2, induced expression of the fusion protein by IPTG; lane 3, supernatant after sonicating; lane 4, pellet after sonicating; lane 5, purified recombinant GST-eIF5C by Glutathione Sepharose 4B; lane 6, cleavage of GST-eIF5C with thrombin for 16hrs; lane 7, gel extraction of recombinant eIF5C. B: Immunoblotting showing the detection of purified recombinant eIF5C and the tissue extracts with the antibody. Lane 1, purified recombinant Ha-eIF5C without GST tag; lane 2, tissue homogenates from 6th instar 120 h larvae. M: standard protein; 12.5% SDS-PAGE.

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