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Figure 3 | BMC Developmental Biology

Figure 3

From: The conserved WW-domain binding sites in Dystroglycan C-terminus are essential but partially redundant for Dystroglycan function

Figure 3

Overexpression of pUASp with MatTubGal4 in germline disrupts the polarity marker Orb. (Orb-red, Dg-green, DAPI – blue, separate channels for Orb are shown on the side of each corresponding picture). A. wild type (wt) stage 4 egg chamber shows normal Orb (red) localization at the posterior side of the oocyte. B. Overexpression of the pUASp-FL transgenic construct disrupts the normal Orb localization. C. Overexpression of the pUASp-2WW does not disrupt normal Orb (red) localization. Similar phenotype is seen with C1-construct that lacks the entire C-terminal region of Dg (D; Fig. 1). E. Overexpression of pUASp-PPSG constructs disrupts oocyte polarity indicated by mislocalization of Orb which has an abnormal side location, F. Percentage of Orb mislocalization as the result of overexpression of different pUASp-Dg constructs. (FL, 49 ± 2, PPSG 44 ± 2, 2WW 19 ± 2, C1 16 ± 3).

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