Figure 2

Overexpression of Dg constructs with mutation in WW binding sites in follicle and germline cells. A, B. Overexpression of 2WW (A) and PPSG (B) constructs in follicle cells marked by GFP. Dg in the wild type cells is expressed at the apical side of the follicle cell epithelium, in contrast to overexpression where Dg is localized in both apical and basal sides (indicated by arrows). C. Overexpression of the constructs in the germline cells.wt – Dg expression in wild type germline cells, MatTubGal4;pUASp-PPSG, nanosGal4/pUASp-PPSG – overexpression of transgenic constructs in germline cells. Both MatTub- and nanosGal4 have distinct expression patterns.