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Figure 1 | BMC Developmental Biology

Figure 1

From: Boundary cells regulate a switch in the expression of FGF3 in hindbrain rhombomeres

Figure 1

Effects of truncated EphA4 on rhombomeres. Flat-mounted dorsal views of chick embryonic hindbrains electroporated unilaterally with dnEphA4-IRES-GFP (B, D, F, G, I, J) or control pCAGGS-GFP (A, C, E, H,) constructs at 6–8 ss, then left to develop for further 24–40 hrs. (A, B): Hindbrains stained with anti-GFP antibody (midline is marked by dashed outlines). (C-D): Binding of ephrinA5-Fc chimera, which occurs in r3/r5, where endogenous Eph receptors are expressed (C, D) and also in ectopic domains corresponding to dnEphA4 electroporated cells (D). (E-G): In situ hybridized embryos using Krox20 probe show normal Krox20 expression in r3/r5 in control hindbrain (E) and ectopic Krox20 expression within r2/r4/r6 territories in dnEphA4-transfected hindbrains (F, G white arrows). (H-J): Confocal imaging of hindbrains stained with anti-EphA4 antibody showing cells expressing endogenous EphA4 within r2/r4/r6 domains on the dnEphA4-expressing side of the hindbrain (I-J, white arrows), compared to controls where EphA4 is localized to r3/r5 (H). Asterisks mark the electroporated side, treatments are stated at the bottom, anterior is at the top and rhombomeres (r) are numbered.

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