Figure 6

Expression of Arm^D88A interferes with redistribution of D E-cadherin during apoptosis. (A, B) th¹⁰⁹ homozygous embryo (stage 9, 60 min post morphogenetic block) expressing Arm^D88A stained for TUNEL (A, A') and expression of the Flag-tagged Arm^D88A transgene (B, B' anti-Flag). (C, D) th¹⁰⁹ homozygous embryo (60 min post cephalic furrow formation) expressing Arm^D88A and stained for Arm (C) and DE-cadherin (D); note persistent localization of DE-cadherin at the plasma membrane. (E-H) DE-cadherin localization in th¹⁰⁹ homozygous embryos with or without expression of Arm^D88A. (E, F) th¹⁰⁹ homozygous embryo (60 min post cephalic furrow formation) expressing Arm^D88A stained for DE-cadherin (E, E'; red in F, F') and TUNEL (green in F, F'). (G, H) th¹⁰⁹ homozygous embryo (60 min post cephalic furrow formation) stained for DE-cadherin (red in H) and TUNEL (green in H). Scale bar in (B) and (F) 40 μm, scale bar in B') and F') 10 μm.