Figure 6
The function of Sds22 is conserved in human cells. The human homologue of Sds22, PPP1R7, was depleted with four siRNA oligos in the human A431 squamous cell carcinoma line. (A) Western blot showing changes in ERM protein phosphorylation and total Ezrin levels in control and PPP1R7 siRNA transfected A431 cells. Bar chart shows fold changes in p-ERM levels normalized to total Ezrin levels in control and PPP1R7 transfected A431 cells. (B) Bar chart shows fold changes in p-S19-MLC levels normalized to total MLC levels in control and PPP1R7 transfected A431 cells. (C) RT-PCR showing beta-actin and PPP1R7 levels in control and PPP1R7 siRNA transfected A431 cells. Bar chart shows % changes in PPP1R7 levels normalized to beta-actin levels. (D) Bar graph showing the average number of cells per cluster in control and PPP1R7 transfected A431 cells. (E) Representative images of p-ERM and p-S19-MLC staining in control and PPP1R7 transfected A431 cells.