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Figure 5 | BMC Developmental Biology

Figure 5

From: Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Figure 5

Sds22 binds to all four Drosophila PP1 proteins and sds22 mutant cells exhibit hyper-phosphorylation of the PP1 targets Sqh/MRLC and Moe. (A) Co-immunoprecipitation of Sds22-GFP with each of the four HA-tagged PP1 isoforms (labelled: 96, 87, 13 and 9) from Drosophila S2 cell lysates. Beads conjugated to anti-HA antibodies were used to precipitate each of the HA-tagged PP1s and Sds22-GFP was found to efficiently co-precipitate. Note that, in the absence of transfected HA-tagged PP1, only a tiny amount of Sds-22 protein precipitates with the HA-beads. (B) Phosphorylated Sqh/MRLC (P-Sqh, red) is elevated and spreads basally in sds22 mutant clones (marked by absence of GFP, green). (C) Levels of total Sqh/MRLC protein (monitored by a Sqh-GFP transgene, green; [27] are not altered in sds22 mutant clones (marked by absence of beta-Gal, red). (D) Phosphorylated Moesin (P-Moe, red) is elevated and spreads basally in sds22 mutant clones (marked by absence of GFP, green). (E) Overall protein levels of Moesin (Moe, red) do not increase in sds22 mutant clones (marked by absence of GFP, green).

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