Figure 5

Morphological and immunophenotypical changes induced by BrdU glial differentiation treatment. A. Morphological changes in SK-N-ER cells (Phase contrast microscopy; 100×). B. GD2 expression levels after 15 days of treatment in I-type cell line SK-N-Be2C. C Calcyclin, (D) Sox10 and (E) S100 expression after 21 days of BrdU treatment in N-type cell line LA1-55N. F. SA-β-GAL positive cells (arrow) after 21 days of BrdU treatment in the I-type SK-N-ER (Phase contrast; 100×). Control cell images are reported in the small squares. Scale bar: 50 μm.