Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos

Table 2 Overview of the integrin RNA expression, relatively compared to the FN1 RNA expression, in bovine oocytes and preimplantation embryos

Gene	Oocyte	2-cell	8-cell	Morula	Blastocyst	Hatched Blastocyst
FN1	+	-	-	+	+++	+++
ITGA2B	+	-	-	-	+	+++
ITGA3	+	+	-	-	+	+++
ITGA5	+	-	-	+	+++	+++
ITGA8	-	-	-	-	+/-	++
ITGAV	+	+/-	-	+	+++	+++
ITGB3	+	-	+/-	++	+++	++
ITGA4	+	+	+	-	-	-
ITGA9	+	+	+	-	-	-
ITGA11	+	+/-	+/-	-	-	-
ITGAD	-	-	-	-	-	+
ITGB1	+++	+++	++	+	+	+
ITGB2	-	-	-	-	-	-
ITGB6	-	-	-	-	-	-
ITGB7	-	-	-	-	-	+

The integrins that follow the FN1 RNA expression pattern (marked in bold) are considered as candidate receptors for the FN1-ESV isoform (-: no expression, +/-: expression in part of the samples; + low expression; ++ moderate expression, +++ high expression compared to FN1 blastocyst expression).

ISSN: 1471-213X