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Table 1 Primer and amplicon information

From: Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos

Primer Target Sequence Amplicon size (bp) Ta
(°C)
BtauFN1
primer pair 1
EIIIB region
Standard curve
5'-CAGAGACGGGCAAGAGAGAG-3'
5'-CAGGTTGGTGAGGGTGATG-3'
+ 1060
- 787
62
BtauFN1
primer pair 2
EIIIA region
Standard curve
5'-GCCCGTTTCCATCAATTACC-3'
5'-TGAGCCGGTCTGCTTGTC-3'
+ 819
- 549
62
BtauFN1
primer pair 3
IIICS region
Standard curve
5'-GCCCTCAAGAACAATCAGAAG-3'
5'-TGAAATGACCACTGCCAAAG-3'
+ 808
- 448
62
BtauFN1
primer pair 4
EIIIB region
Full-length region
5'-CATGCCGATCAGAGTTCCT-3'
5'-AAGAGTTTAGCGGGGTCCA-3'
202 62
BtauFN1
primer pair 5
EIIIA region
Full-length region
5'-GGACCATCGAAAACGAAAAC-3'
5'-GGAATCGACATCCACATCAG-3'
195 62
BtauFN1
primer pair 6
IIICS region
Full-length region
5'-AGGGGAGACGTAGACCATCA-3'
5'-GGCACTAGCAGAGGTTCCAG-3'
201 62
BtauFN1
primer pair 7
EIIIB region
Boundary spanning
5'-CATGCCGATCAGAGTTCCTG-3'
5'-GAGGGACAGCTGGGATGATG-3'
130 62
BtauFN1
primer pair 8
EIIIA region
Boundary spanning
5'-GGTAACCACCATTCCTGCAC-3'
5'-CCTGATACAACCACGGATGA-3'
189 62
BtauFN1
primer pair 9
IIICS region
Boundary spanning
5'-GGAGGAAAAAGACAGGCCAAG-3'
5'-GGCACTAGCAGAGGTTCCAG-3'
151 62
BtauFN1
primer pair 10
Conserved region
XM_864390
5'-ACTGCCCACTCCTACAACCA-3'
5'-TCTGCGAACACCACTCCA-3'
196 62
BtauITGA2B Integrin αIIb
NM_001014929
5'-TGGAAACACCCACGTCCA-3'
5'-CCAGCTTCTCCACGCTCAC-3'
155 60
BtauITGA3 Integrin α3
NM_001101900
5'-GGCAGACCTGAACAATGATG-3'
5'-CAATGCTTGCCACAGAGAAG-3'
191 61
BtauITGA4 Integrin α4
NM_174748
5'-AGACGAGGATCTCAACATCACA-3'
5'-AATGAGGCTGGACTTACAAACC-3'
147 60
BtauITGA5 Integrin α5
XM_614854
5'-GTGTGAGGCCGTGTATGAAG-3'
5'-CAGACCGAGGAGCAGGAG-3'
178 60
BtauITGA8 Integrin α8
XR_027842
5'-GCTCAAGTAGAAATAAGAGGAGT
GTC-3' 5'-AGGAGGGTGTCGCTGATG-3'
167 60
BtauITGA9 Integrin α9
XM_610637
5'-ATGTGACGGGAGAGGAGGAG-3'
5'-CGAGGTTGGAGATGGAGATG-3'
243 61
BtauITGA11 Integrin α11
XM_602058
5'-CGCCAGATCACGTTTGAGA-3'
5'-ACTCCAGCACTTTGGGTGTG-3'
190 60
BtauITGAD Integrin αD
NM_001102496
5'-GACTGCTCCATCGCTGACT-3'
5'-CACGACCAATGTCTTCTTCTG-3'
141 60
BtauITGAV Integrin αV
NM_174367
5'-GGGACTTCCAGACCATCAAG-3'
5'-GTCTATATCCGTGGCTCCTTTC-3'
275 61
BtauITGB1 Integrin β1
NM_174368
5'-TGAGGTGAACAGCGAAGACA-3'
5'-TTGCACTCACACACTCGACA-3'
231 60
BtauITGB2 Integrin β2
NM_175781
5'-GGCAGAAAGCAACATCCAG-3'
5'-TGATAAGCTCCACCACGTTC-3'
137 60
BtauITGB3 Integrin β3
XM_616376
5'-TTCTCCACCAGAGGCTATCA-3'
5'-TCTGCTTCTTCACCTCCTCA-3'
131 61
BtauITGB6 Integrin β6
NM_174698
5'-ACAACGGCAATGGTTCCTTC-3'
5'-GCTCCTGCACACGCACTC-3'
299 60
BtauITGB7 Integrin β7
NM_001105365
5'-CTACAGTCGCAGCCCAGAG-3'
5'-CCGGAGGGAGAAGAGAGTG-3'
260 60