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Table 1 Primer and amplicon information
| Primer | Target | Sequence | Amplicon size (bp) |
Ta (°C) |
|---|---|---|---|---|
|
BtauFN1 primer pair 1 |
EIIIB region Standard curve |
5'-CAGAGACGGGCAAGAGAGAG-3' 5'-CAGGTTGGTGAGGGTGATG-3' |
+ 1060 - 787 | 62 |
|
BtauFN1 primer pair 2 |
EIIIA region Standard curve |
5'-GCCCGTTTCCATCAATTACC-3' 5'-TGAGCCGGTCTGCTTGTC-3' |
+ 819 - 549 | 62 |
|
BtauFN1 primer pair 3 |
IIICS region Standard curve |
5'-GCCCTCAAGAACAATCAGAAG-3' 5'-TGAAATGACCACTGCCAAAG-3' |
+ 808 - 448 | 62 |
|
BtauFN1 primer pair 4 |
EIIIB region Full-length region |
5'-CATGCCGATCAGAGTTCCT-3' 5'-AAGAGTTTAGCGGGGTCCA-3' | 202 | 62 |
|
BtauFN1 primer pair 5 |
EIIIA region Full-length region |
5'-GGACCATCGAAAACGAAAAC-3' 5'-GGAATCGACATCCACATCAG-3' | 195 | 62 |
|
BtauFN1 primer pair 6 |
IIICS region Full-length region |
5'-AGGGGAGACGTAGACCATCA-3' 5'-GGCACTAGCAGAGGTTCCAG-3' | 201 | 62 |
|
BtauFN1 primer pair 7 |
EIIIB region Boundary spanning |
5'-CATGCCGATCAGAGTTCCTG-3' 5'-GAGGGACAGCTGGGATGATG-3' | 130 | 62 |
|
BtauFN1 primer pair 8 |
EIIIA region Boundary spanning |
5'-GGTAACCACCATTCCTGCAC-3' 5'-CCTGATACAACCACGGATGA-3' | 189 | 62 |
|
BtauFN1 primer pair 9 |
IIICS region Boundary spanning |
5'-GGAGGAAAAAGACAGGCCAAG-3' 5'-GGCACTAGCAGAGGTTCCAG-3' | 151 | 62 |
|
BtauFN1 primer pair 10 |
Conserved region XM_864390 |
5'-ACTGCCCACTCCTACAACCA-3' 5'-TCTGCGAACACCACTCCA-3' | 196 | 62 |
| BtauITGA2B |
Integrin αIIb NM_001014929 |
5'-TGGAAACACCCACGTCCA-3' 5'-CCAGCTTCTCCACGCTCAC-3' | 155 | 60 |
| BtauITGA3 |
Integrin α3 NM_001101900 |
5'-GGCAGACCTGAACAATGATG-3' 5'-CAATGCTTGCCACAGAGAAG-3' | 191 | 61 |
| BtauITGA4 |
Integrin α4 NM_174748 |
5'-AGACGAGGATCTCAACATCACA-3' 5'-AATGAGGCTGGACTTACAAACC-3' | 147 | 60 |
| BtauITGA5 |
Integrin α5 XM_614854 |
5'-GTGTGAGGCCGTGTATGAAG-3' 5'-CAGACCGAGGAGCAGGAG-3' | 178 | 60 |
| BtauITGA8 |
Integrin α8 XR_027842 |
5'-GCTCAAGTAGAAATAAGAGGAGT GTC-3' 5'-AGGAGGGTGTCGCTGATG-3' | 167 | 60 |
| BtauITGA9 |
Integrin α9 XM_610637 |
5'-ATGTGACGGGAGAGGAGGAG-3' 5'-CGAGGTTGGAGATGGAGATG-3' | 243 | 61 |
| BtauITGA11 |
Integrin α11 XM_602058 |
5'-CGCCAGATCACGTTTGAGA-3' 5'-ACTCCAGCACTTTGGGTGTG-3' | 190 | 60 |
| BtauITGAD |
Integrin αD NM_001102496 |
5'-GACTGCTCCATCGCTGACT-3' 5'-CACGACCAATGTCTTCTTCTG-3' | 141 | 60 |
| BtauITGAV |
Integrin αV NM_174367 |
5'-GGGACTTCCAGACCATCAAG-3' 5'-GTCTATATCCGTGGCTCCTTTC-3' | 275 | 61 |
| BtauITGB1 |
Integrin β1 NM_174368 |
5'-TGAGGTGAACAGCGAAGACA-3' 5'-TTGCACTCACACACTCGACA-3' | 231 | 60 |
| BtauITGB2 |
Integrin β2 NM_175781 |
5'-GGCAGAAAGCAACATCCAG-3' 5'-TGATAAGCTCCACCACGTTC-3' | 137 | 60 |
| BtauITGB3 |
Integrin β3 XM_616376 |
5'-TTCTCCACCAGAGGCTATCA-3' 5'-TCTGCTTCTTCACCTCCTCA-3' | 131 | 61 |
| BtauITGB6 |
Integrin β6 NM_174698 |
5'-ACAACGGCAATGGTTCCTTC-3' 5'-GCTCCTGCACACGCACTC-3' | 299 | 60 |
| BtauITGB7 |
Integrin β7 NM_001105365 |
5'-CTACAGTCGCAGCCCAGAG-3' 5'-CCGGAGGGAGAAGAGAGTG-3' | 260 | 60 |
