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Table 1 Primer and amplicon information

From: Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos

Primer Target Sequence Amplicon size (bp) Ta (°C)
BtauFN1 primer pair 1 EIIIB region Standard curve 5'-CAGAGACGGGCAAGAGAGAG-3' 5'-CAGGTTGGTGAGGGTGATG-3' + 1060 - 787 62
BtauFN1 primer pair 2 EIIIA region Standard curve 5'-GCCCGTTTCCATCAATTACC-3' 5'-TGAGCCGGTCTGCTTGTC-3' + 819 - 549 62
BtauFN1 primer pair 3 IIICS region Standard curve 5'-GCCCTCAAGAACAATCAGAAG-3' 5'-TGAAATGACCACTGCCAAAG-3' + 808 - 448 62
BtauFN1 primer pair 4 EIIIB region Full-length region 5'-CATGCCGATCAGAGTTCCT-3' 5'-AAGAGTTTAGCGGGGTCCA-3' 202 62
BtauFN1 primer pair 5 EIIIA region Full-length region 5'-GGACCATCGAAAACGAAAAC-3' 5'-GGAATCGACATCCACATCAG-3' 195 62
BtauFN1 primer pair 6 IIICS region Full-length region 5'-AGGGGAGACGTAGACCATCA-3' 5'-GGCACTAGCAGAGGTTCCAG-3' 201 62
BtauFN1 primer pair 7 EIIIB region Boundary spanning 5'-CATGCCGATCAGAGTTCCTG-3' 5'-GAGGGACAGCTGGGATGATG-3' 130 62
BtauFN1 primer pair 8 EIIIA region Boundary spanning 5'-GGTAACCACCATTCCTGCAC-3' 5'-CCTGATACAACCACGGATGA-3' 189 62
BtauFN1 primer pair 9 IIICS region Boundary spanning 5'-GGAGGAAAAAGACAGGCCAAG-3' 5'-GGCACTAGCAGAGGTTCCAG-3' 151 62
BtauFN1 primer pair 10 Conserved region XM_864390 5'-ACTGCCCACTCCTACAACCA-3' 5'-TCTGCGAACACCACTCCA-3' 196 62
BtauITGA2B Integrin αIIb NM_001014929 5'-TGGAAACACCCACGTCCA-3' 5'-CCAGCTTCTCCACGCTCAC-3' 155 60
BtauITGA3 Integrin α3 NM_001101900 5'-GGCAGACCTGAACAATGATG-3' 5'-CAATGCTTGCCACAGAGAAG-3' 191 61
BtauITGA4 Integrin α4 NM_174748 5'-AGACGAGGATCTCAACATCACA-3' 5'-AATGAGGCTGGACTTACAAACC-3' 147 60
BtauITGA5 Integrin α5 XM_614854 5'-GTGTGAGGCCGTGTATGAAG-3' 5'-CAGACCGAGGAGCAGGAG-3' 178 60
BtauITGA8 Integrin α8 XR_027842 5'-GCTCAAGTAGAAATAAGAGGAGT GTC-3' 5'-AGGAGGGTGTCGCTGATG-3' 167 60
BtauITGA9 Integrin α9 XM_610637 5'-ATGTGACGGGAGAGGAGGAG-3' 5'-CGAGGTTGGAGATGGAGATG-3' 243 61
BtauITGA11 Integrin α11 XM_602058 5'-CGCCAGATCACGTTTGAGA-3' 5'-ACTCCAGCACTTTGGGTGTG-3' 190 60
BtauITGAD Integrin αD NM_001102496 5'-GACTGCTCCATCGCTGACT-3' 5'-CACGACCAATGTCTTCTTCTG-3' 141 60
BtauITGAV Integrin αV NM_174367 5'-GGGACTTCCAGACCATCAAG-3' 5'-GTCTATATCCGTGGCTCCTTTC-3' 275 61
BtauITGB1 Integrin β1 NM_174368 5'-TGAGGTGAACAGCGAAGACA-3' 5'-TTGCACTCACACACTCGACA-3' 231 60
BtauITGB2 Integrin β2 NM_175781 5'-GGCAGAAAGCAACATCCAG-3' 5'-TGATAAGCTCCACCACGTTC-3' 137 60
BtauITGB3 Integrin β3 XM_616376 5'-TTCTCCACCAGAGGCTATCA-3' 5'-TCTGCTTCTTCACCTCCTCA-3' 131 61
BtauITGB6 Integrin β6 NM_174698 5'-ACAACGGCAATGGTTCCTTC-3' 5'-GCTCCTGCACACGCACTC-3' 299 60
BtauITGB7 Integrin β7 NM_001105365 5'-CTACAGTCGCAGCCCAGAG-3' 5'-CCGGAGGGAGAAGAGAGTG-3' 260 60