Table 1 Primer and amplicon information
Primer | Target | Sequence | Amplicon size (bp) | Ta (°C) |
|---|---|---|---|---|
BtauFN1 primer pair 1 | EIIIB region Standard curve | 5'-CAGAGACGGGCAAGAGAGAG-3' 5'-CAGGTTGGTGAGGGTGATG-3' | + 1060 - 787 | 62 |
BtauFN1 primer pair 2 | EIIIA region Standard curve | 5'-GCCCGTTTCCATCAATTACC-3' 5'-TGAGCCGGTCTGCTTGTC-3' | + 819 - 549 | 62 |
BtauFN1 primer pair 3 | IIICS region Standard curve | 5'-GCCCTCAAGAACAATCAGAAG-3' 5'-TGAAATGACCACTGCCAAAG-3' | + 808 - 448 | 62 |
BtauFN1 primer pair 4 | EIIIB region Full-length region | 5'-CATGCCGATCAGAGTTCCT-3' 5'-AAGAGTTTAGCGGGGTCCA-3' | 202 | 62 |
BtauFN1 primer pair 5 | EIIIA region Full-length region | 5'-GGACCATCGAAAACGAAAAC-3' 5'-GGAATCGACATCCACATCAG-3' | 195 | 62 |
BtauFN1 primer pair 6 | IIICS region Full-length region | 5'-AGGGGAGACGTAGACCATCA-3' 5'-GGCACTAGCAGAGGTTCCAG-3' | 201 | 62 |
BtauFN1 primer pair 7 | EIIIB region Boundary spanning | 5'-CATGCCGATCAGAGTTCCTG-3' 5'-GAGGGACAGCTGGGATGATG-3' | 130 | 62 |
BtauFN1 primer pair 8 | EIIIA region Boundary spanning | 5'-GGTAACCACCATTCCTGCAC-3' 5'-CCTGATACAACCACGGATGA-3' | 189 | 62 |
BtauFN1 primer pair 9 | IIICS region Boundary spanning | 5'-GGAGGAAAAAGACAGGCCAAG-3' 5'-GGCACTAGCAGAGGTTCCAG-3' | 151 | 62 |
BtauFN1 primer pair 10 | Conserved region XM_864390 | 5'-ACTGCCCACTCCTACAACCA-3' 5'-TCTGCGAACACCACTCCA-3' | 196 | 62 |
BtauITGA2B | Integrin αIIb NM_001014929 | 5'-TGGAAACACCCACGTCCA-3' 5'-CCAGCTTCTCCACGCTCAC-3' | 155 | 60 |
BtauITGA3 | Integrin α3 NM_001101900 | 5'-GGCAGACCTGAACAATGATG-3' 5'-CAATGCTTGCCACAGAGAAG-3' | 191 | 61 |
BtauITGA4 | Integrin α4 NM_174748 | 5'-AGACGAGGATCTCAACATCACA-3' 5'-AATGAGGCTGGACTTACAAACC-3' | 147 | 60 |
BtauITGA5 | Integrin α5 XM_614854 | 5'-GTGTGAGGCCGTGTATGAAG-3' 5'-CAGACCGAGGAGCAGGAG-3' | 178 | 60 |
BtauITGA8 | Integrin α8 XR_027842 | 5'-GCTCAAGTAGAAATAAGAGGAGT GTC-3' 5'-AGGAGGGTGTCGCTGATG-3' | 167 | 60 |
BtauITGA9 | Integrin α9 XM_610637 | 5'-ATGTGACGGGAGAGGAGGAG-3' 5'-CGAGGTTGGAGATGGAGATG-3' | 243 | 61 |
BtauITGA11 | Integrin α11 XM_602058 | 5'-CGCCAGATCACGTTTGAGA-3' 5'-ACTCCAGCACTTTGGGTGTG-3' | 190 | 60 |
BtauITGAD | Integrin αD NM_001102496 | 5'-GACTGCTCCATCGCTGACT-3' 5'-CACGACCAATGTCTTCTTCTG-3' | 141 | 60 |
BtauITGAV | Integrin αV NM_174367 | 5'-GGGACTTCCAGACCATCAAG-3' 5'-GTCTATATCCGTGGCTCCTTTC-3' | 275 | 61 |
BtauITGB1 | Integrin β1 NM_174368 | 5'-TGAGGTGAACAGCGAAGACA-3' 5'-TTGCACTCACACACTCGACA-3' | 231 | 60 |
BtauITGB2 | Integrin β2 NM_175781 | 5'-GGCAGAAAGCAACATCCAG-3' 5'-TGATAAGCTCCACCACGTTC-3' | 137 | 60 |
BtauITGB3 | Integrin β3 XM_616376 | 5'-TTCTCCACCAGAGGCTATCA-3' 5'-TCTGCTTCTTCACCTCCTCA-3' | 131 | 61 |
BtauITGB6 | Integrin β6 NM_174698 | 5'-ACAACGGCAATGGTTCCTTC-3' 5'-GCTCCTGCACACGCACTC-3' | 299 | 60 |
BtauITGB7 | Integrin β7 NM_001105365 | 5'-CTACAGTCGCAGCCCAGAG-3' 5'-CCGGAGGGAGAAGAGAGTG-3' | 260 | 60 |