Figure 5

Examination of cellular ultrastructure in vari^48EPmutants. (A-D) We have compared the ultrastructure of the cells of stage 16 trachea and hindgut between wildtype (A, C) and vari^48EPmutants (B, D). The lumen of vari trachea could be significantly dilated relative to wildtype, but the structure of lumenal cuticle and tanideal ridges were not affected (arrow B). We also examined the ultrastructure of epithelial cell junctions from semi-serial sections of the hindgut. Relative to wildtype (C), apposed lateral membranes (arrows) and septate junctions (arrowheads) were similar in vari mutants (D). Calibration: 2 μm A, B and 200 nm C and D.