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Figure 3 | BMC Developmental Biology

Figure 3

From: Varicose: a MAGUK required for the maturation and function of Drosophila septate junctions

Figure 3

Varicose nervous system expression is novel for a MAGUK. (A-D) Stage 16–17 wildtype embryos were stained with Vari and glial cell marker, Repo. Vari localizes with Repo in peripheral glial cells (arrow, A) in a similar pattern to NrxIV, a known pSJ marker of PNS glia (arrow, B). Embryos labeled with Vari and NrxIV show co-localization of these proteins in peripheral glia (arrow, C) but not in midline glia (asterisk, C). No Vari labeling was found in the CNS (at left of A, asterisk, C-D). NrxIV and Vari label at right is in the ectoderm. Vari does not label developing synapses identified using Fasciclin II antibody (arrow, D). Ventral views from a single confocal section. Anterior at top; ventral midline at left. Brains from late 3rd instar larva were labeled with Vari and Dlg (E-G). (E) Vari expression is concentrated in the apical region (arrow) of neuroepithelial (NE) cells (right of arrowheads) and excluded from neuroblasts (NBs) (left of arrowheads). (F) Dlg is concentrated at the SJ of NE cells (arrow) and distributed cortically in NBs. Co-localization of Dlg and Vari is limited along the lateral membrane of the NE (G). (H-K) Vari (green) is detected throughout the cytoplasm in a subset of central brain neuroblasts. Vari fails to co-localize with Dlg at the cortex (red, H) and appears to have low levels of expression in Elav expressing post-mitotic neurons (arrow, Elav, I). Vari is excluded from ganglion mother cells that label for Prospero (red, J), and glial cells that label for Repo (red, K) (NBs denoted by asterisk). (L-O) Pupal brains stained with Vari (green) and Dlg (L), FasII (M), Elav (N) or Repo (O) (red). (L, N, O) Single cross sections visualized by confocal microscopy. Vari and Dlg co-localize in cell bodies (inset, L) but fail to co-localize in the antennal lobes (AL) and mushroom bodies (MB). (M) A projection of sections visualized by confocal microscopy. Vari is excluded from the MB and axon tracts labeled by FasII. Vari expression appears to concentrate in areas surrounding the axon tracts (inset, M). Vari localizes to cells expressing Elav (inset, N) but is not expressed in glial cells labeled with Repo (inset, O). Calibration: 20 μm, A-D and E-G; 10 μm, H-K; 50 μm, L-O.

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