Figure 2

Varicose is detected at the septate junction in ectodermally-derived tissues. (A) WT embryos stained with Vari (green) and tracheal antibody, 2A12 (red). Vari is restricted to epithelial cells and is excluded from tracheal lumen (arrow). (B-C) WT embryonic hindgut (B) and epidermis (C) labeled with Vari (green) and nuclear stain, propidium iodide (red). Vari is limited to the basolateral region of the plasma membrane in both tissues. (D) Vari was not detected at the SJ in the null allele, vari^48EP. (E-G) Confocal microscopy was used to visualize the hindgut of WT embryos, labeled with Vari (green) and SJ markers (red). Vari is restricted to SJs, shown by co-localization with SJ markers FasIII (E), NrxIV (F), and Na⁺K⁺ATPase (G). Apical membrane faces are indicated (a) and basal (b). All embryos are stage 15. WT, wildtype. Calibration: 5 μm, A; 2 μm, B-G.