From: Deaf-1 regulates epithelial cell proliferation and side-branching in the mammary gland
RT-PCR | |||
---|---|---|---|
Transcript | Sequence (5'-3') | Annealing Temp (x°C) | Amplicon size (bp) |
Rac3 | Â | Â | Â |
forward | GATGGTGGATGGGAAGCCAGTTAAC | 68 | 300 |
reverse | GGATGGCCTCGTCGAACACTGTC | Â | Â |
HPRT | Â | Â | Â |
forward | CACAGGACTAGAACACCTGC | 65 (+ 5% glycerol) | 229 |
reverse | GCTGGTGAAAAGGACCTCT | Â | Â |
Quantitative real-time PCR | |||
Transcript | Sequence (5'-3') | Â | Amplicon size (bp) |
Deaf-1 | Â | Â | Â |
forward | AGAATGAGCTGCCCACAACT | Â | 133 |
reverse | TCAAAGGTCAGTGCTCCAGA | Â | Â |
Rac3 | Â | Â | Â |
forward | CACACACACCCATCCTTCTG | Â | 100 |
reverse | TAGGTTATGGGTGCCAGCTT | Â | Â |
18S rRNA | Â | Â | Â |
forward | GTAACCCGTTGAACCCCATT | Â | 152 |
reverse | CCATCCAATCGGTAGTAGCG | Â | Â |
CK18 | Â | Â | Â |
forward | CAAGATCATCGAAGACCTGAGGC | Â | 384 |
reverse | TGTTCATACTGGGCACGGATGTCC | Â | Â |