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Table 3 Primers for RT-PCR and quantitative real-time-PCR analysis

From: Deaf-1 regulates epithelial cell proliferation and side-branching in the mammary gland

RT-PCR

Transcript

Sequence (5'-3')

Annealing Temp (x°C)

Amplicon size (bp)

Rac3

   

forward

GATGGTGGATGGGAAGCCAGTTAAC

68

300

reverse

GGATGGCCTCGTCGAACACTGTC

  

HPRT

   

forward

CACAGGACTAGAACACCTGC

65 (+ 5% glycerol)

229

reverse

GCTGGTGAAAAGGACCTCT

  

Quantitative real-time PCR

Transcript

Sequence (5'-3')

 

Amplicon size (bp)

Deaf-1

   

forward

AGAATGAGCTGCCCACAACT

 

133

reverse

TCAAAGGTCAGTGCTCCAGA

  

Rac3

   

forward

CACACACACCCATCCTTCTG

 

100

reverse

TAGGTTATGGGTGCCAGCTT

  

18S rRNA

   

forward

GTAACCCGTTGAACCCCATT

 

152

reverse

CCATCCAATCGGTAGTAGCG

  

CK18

   

forward

CAAGATCATCGAAGACCTGAGGC

 

384

reverse

TGTTCATACTGGGCACGGATGTCC

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