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Figure 1 | BMC Developmental Biology

Figure 1

From: Evidence for Hox-specified positional identities in adult vasculature

Figure 1

Analysis of Hoxc11-lacZ reporter gene expression in hindlimb vasculature. (A) E12 embryo of the transgenic shows posteriorly restricted lacZ reporter gene expression (blue) in most of the hindlimb region and tailbud, as well as in the posterior zeugopodal forelimb region. (B) X-Gal staining of posterior trunk and hindlimbs of 0.5 d newborn Hoxc11-lacZ transgenic mouse after skinning reveals expression in skeletal muscles and blood vessels (yellow arrow). (C) Lateral view of stained hindlimb of 42 d Hoxc11-lacZ transgenic mouse shows conspicuous β-gal activity in sciatic artery. (D-F) Medial view of same hindlimb as shown in panel C is presented in panel E and reveals distally restricted expression in both arteries and veins; close-up views of upper and lower sections of femoral artery (red arrows) and vein (blue arrows) are shown in panels D and F, respectively. (G) Lateral view of stained hindlimb from 1 yr old Hoxc11-lacZ transgenic mouse shows persistent reporter gene expression in blood vessels, most prominently in sciatic artery. All mice were derived from transgenic line TG(Hoxc11-lacZ)62D9Awg; fl: forelimb; hl: hindlimb; tlb: tailbud.

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