Figure 9
From: Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells
Laminin stimulates hESC-derived neural progenitor expansion and neuronal neurite outgrowth in a dose-dependent manner. Dark EB-derived neural populations at 3 days postplating on PDL/laminin substrates with 5 different concentrations: 1 μg/ml, 10 μg/ml, 30 μg/ml, 60 μg/ml and 100 μg/ml. The cultures were double-immunostained for nestin and TuJ1. (A, B) Bar plots summarizing the responses of number of nestin+ neural progenitors (A) and total neurite length of TuJ1+ neurons (B) to different laminin concentrations. The neural progenitor expansion and total neurite length of neurons per cell increases with concentrations and reach a peak at 60 μg/ml. Values are expressed as mean ± SEM of 3 independent experiments. Statistical differences for cells/mm2 and total neurite length per cell between 60 μg/ml or 100 μg/ml and 1 μg/ml or 10 μg/ml or 30 μg/ml are significant * p < 0.05; ** p < 0.01.