Neurite outgrowth of hESC-derived neurons are greater on PDL/laminin and matrigel substrates than other substrates. Light EBs were cultured on 5 different substrates: PDL, PDL/laminin, PDL/fibronectin, collagen and Matrigel for 3 days in the NDM and immunostained for TuJ1. (A) A panel of immunofluorescent images showing representative fields of TuJ1+ cells expanded on PDL, PDL/fibronectin (PDL/FN), PDL/laminin (PDL/LN), collagen and Matrigel for 3 days. (B, C) Bar plots summarizing the effect of different substrates on number of neuritis (B) and total neurite length (C) per cell. The laminin (LN)- and matrigel (MG)-induced number of neuritis and total neurite length per cell are significantly higher than on PDL, fibronectin (FN), and collagen (CG). Values are expressed as mean ± SEM of 4 independent experiments. Statistical differences for number of neuritis and for total neurite length per cell between LM or MG and PDL or FN or CG are significant ** p < 0.01. Scale bar in (A) = 30 μm.