hESC-derived neural progenitors are highly proliferative and multipotent. (A-C) Double-immunostaining of differentiated cells at 2 days postplating for nestin (red) and proliferative marker BrdU (greed), indicating approximately 68% of nestin+ cells are BrdU incorporated. (D-F) Double-immunostaining for nestin (red) and A2B5 (green). (G-I) Double-immunostaining for nestin (red) and PS-NCAM (green). The overlapping of nestin and A2B5 or PS-NCAM indicates that hESC-derived nestin+ neural progenitors also express other neural differentiating markers. (J-M) Immunostaining of progeny of hESC-derived neural progenitors for mature neuronal marker MAP2 (J), GABAergic neuronal marker GAD65/67 (K), astrocytic marker GFAP (L) and O4, developing oligodentrocyte marker (M). Scale bars: (A) = 200 μm; (D) = 100 μm; (G) = 100 μm; (J-M) = 30 μm.