Figure 5
From: Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells
Neural rosettes generated on collagen (A-D) and matrigel (E-H) substrates. (A-D) A TE03-derived light EB at 3 days postplating on type I collagen substrates was triple-immunostained for nestin (B), TuJ1 (C) and Sox1 (blue in D). Note that TuJ1 stains radial configurations of structures in rosettes (pointed by two solid arrows) and a cluster of neurons (pointed by a dished arrow) in (C). Scale bar: 100 μm. (E-H) Neural rosettes, neural progenitors and neurons are developed in a TE03-derived light EB cultured on matrigel for 2 days. A solid arrow in (E) points to a TuJ1-labeld radial arrangement (G) which overlaps a rosette in phase contrast image (E). The dashed arrow in (G) indicates TuJ1+ neurons with long processes. The majority of cells in the EB are nestin+ (F). Scale bars in (A) and (E) = 100 μm.