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Figure 3 | BMC Developmental Biology

Figure 3

From: Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints

Figure 3

Immunolocalization of Dnmt1o and Dnmt1s proteins in mouse embryonic fibroblasts and 1-cell embryos. A. Immunostaining of primary embryonic fibroblasts from either heterozygous Dnmt1V/+ (V/+) or homozygous Dnmt1V/V(V/V) mice using the UPT82 antiserum. Intracellular DNA was detected with DAPI. B. Immunostaining of MII oocytes and pronuclear-stage embryos of different genotypes with the UPT82 antiserum. PN3 and PN4 refer to pronuclear stage 3 and 4 as defined by morphologic criteria [17]. Insets are bright-field images of immunostained embryos. C. Immunostaining of pronuclear-stage embryos with the UPTC21 antiserum to detect the abundant Dnmt1o protein. Genotype abbreviations are the same as in the legends to Figures 1 and 2. Bar, 20 μm.

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