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Figure 1 | BMC Developmental Biology

Figure 1

From: Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints

Figure 1

Dnmt1o and Dnmt1s forms of Dnmt1 protein and their expression in oocytes. A. Schematic representation of the mouse Dnmt1s and Dnmt1o proteins. Near the N-termini two regions are highlighted, the PCNA binding domain (PBD) and targeting sequence (TS) that have been implicated in targeting Dnmt1 to replication foci [18,29]. The black rectangles identify the positions of the mouse Dnmt1s amino acid sequences used to immunize a rabbit to produce the UPT82 antiserum or to immunize a chicken to produce the UPTC21 antiserum [6]. B. Immunoblots showing expression of Dnmt1s protein in oocytes from various mouse lines. The upper and lower blots were probed with the UPT82 antiserum. + refers to wild-type oocytes; 1s/1o refers to homozygous mutant oocytes from the Dnmt11s/1ostrain [6]; Δ1o refers to homozygous mutant oocytes from the Dnmt1Δ1ostrain; TgEL/+ refers to oocytes from hemizygous TgEL transgenic mice. Sample sizes for the upper blot: 100 wild-type GV oocytes; 120 wild-type MII oocytes; 25 1s/1o oocytes; 60 Δ1o oocytes. Sample sizes for the lower blot: 105 wild-type MII oocytes; 40 TgEL/+ oocytes. C. Expression of Dnmt1o protein in wild-type, 1s/1o and Δ1o oocytes. The same blot shown in panel B was probed with UPTC21.

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