Figure 9
From: Generation of diverse neuronal subtypes in cloned populations of stem-like cells
NE-4C derived neurons can acquire different neurotransmitter phenotypes. A: Double immune-staining for MAP2 (red; A1, A3) neuronal marker and for VGAT (green; A2, A3) GABAergic neuronal marker showed that VGAT staining was confined to neurons, but not all neurons displayed VGAT-immune-reactivity. Arrows mark double immune-reactive cells; asterisk indicates a VGAT-negative neuron. B: Double immune-staining for βIII-tubulin (green; B1, B3) and VGLUT2 (red; B2, B3) glutamatergic neuronal marker demonstrated the presence of both glutamatergic (arrows) and non-glutamatergic neurons. Asterisk marks βIII-tubulin positive but VGLUT2 negative neurons. C: Double staining for VGAT (green; C2, C3) and VGLUT2 (red; C1, C3) revealed a complete segregation of GABAergic (arrows) and glutamatergic (arrowheads) processes. Immunostaining was made on differentiated NE-4C cultures at stage 6. Bars: 10 μm