Figure 3
From: Generation of diverse neuronal subtypes in cloned populations of stem-like cells
Differentiated (stage 6) NE-4C cultures contain neurons and astrocytes, but populations of non-differentiated cells persist. Phase contrast view (A) shows the arrangement of neurons on the top of a substrate-attached monolayer of non-neuronal cells. SSEA-1 positive non-differentiated cells (red on B) are located in the substrate-attached monolayer beneath the network of βIII-tubulin (green) immunoreactive neurons. GFAP positive astroglial cells (red on C) appear in the substrate-attached monolayer and inside the loose neuronal aggregates among βIII-tubulin (green) immunoreactive neurons. In stage 6, morphologically mature neurons (D) develop in the vicinity of developing and mature glial cells stained for the GLAST glutamate transporter (green). Bars: 20 μm for A, 50 μm for B and 10 μm for C and D