Figure 5
From: Transcriptional regulation of mouse alpha A-crystallin gene in a 148kb Cryaa BAC and its derivates
Expression of EGFP from the 15 kb Cryaa/EGFP fragment in lens. EGFP expression is first evident at E10.5 in the lens pit (A, F) and becomes expressed throughout the lens vesicle at E11.5 (B, G). As fiber cell differentiation commences at E12.5 (C, H), intense EGFP expression is observed. Prominent expression is also evident in the overlaying lens epithelium (C, H). EGFP expression continues to be highly expressed both in the lens fiber and epithelial cells of the developing E14.5 (D, I) and PND1 lens (E, J). Nuclear DAPI staining is blue, and the cytoskeletal phalloidin staining is red. Lens epithelial cells, e; lens fiber cells, f; lens pit, lp; lens vesicle, lv. Scale bar = 100 μm.