Figure 2
From: Transcriptional regulation of mouse alpha A-crystallin gene in a 148kb Cryaa BAC and its derivates
Schematic representation of three αA-crystallin transgene constructs. (A) A schematic diagram of the mouse Cryaa locus (chromosome 17) and its adjacent loci, U2af1 and Snf1lk. The 148 kb BAC Clone RP-23-465G4 is shown in red. The XmaI-SpeI sites delineate 15 kb of the Cryaa locus. Exons in the Cryaa gene (black box), DCR1 and DCR3 (orange box), centromere, cen; telomere, tel; Exon, ex; rodent specific Cryaa exon, Ins. (B) Modification of αA-BAC using the λ prophage system for homologous recombination [25]. (C) Generation of αA-BAC(ΔDCR3) using a shuttle vector [26]. (D) Diagrammatic representation of the 15 kb portion of the mouse αA-crystallin locus with an EGFP insert (see panel (C)) marked by unique XmaI and SpeI restriction sites.