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Figure 2 | BMC Developmental Biology

Figure 2

From: An in vivo reporter of BMP signaling in organogenesis reveals targets in the developing kidney

Figure 2

Comparison of BRE-lacZ with nuclear accumulation of phosphorylated SMAD 1, 5 and 8. A. In general, immunostaining for nuclear accumulation of phosphorylated forms of SMADS 1, 5 and 8 (pSMAD1/5/8) is weak, but strongest signals are seen in forebrain, snout, dorsal root ganglia, neural tube and kidney. B. At E12.5, intense reporter gene activation can be seen in areas overlapping with nuclear pSMAD1/5/8 staining: forebrain, snout, dorsal root ganglia, ventral neural tube, kidney and heart. C. At E10.5, reporter gene activation is intense in heart, forebrain, snout, neural tube, dorsal root ganglia and dorsal aorta as well as blood vessels of the head. D. Vibratome section of BRE-lacZ head showing strong signal in trigeminal ganglion (TrG) and snout (Sn). E. Abundant nuclear pSMAD1/5/8 is seen in the trigeminal ganglion. F. Nuclear pSMAD1/5/8 colocalizes with BRE-lacZ expression in the snout. G. Nuclear pSMAD1/5/8 in forebrain. H, I. Comparison between BRE-lacZ and pSMAD1/5/8 in the eye shows significant overlap in the corneal epithelial layer and lens (insets). J, K. β-galactosidase and nuclear pSMAD1/5/8 overlap in cells of the dorsal root ganglion. L, M. BMP signaling can be detected by both BRE-lacZ and pSMAD1/5/8 in the ventral and dorsal neural tube (square brackets, insets), but not in the intermediate region. Abbreviations: DA: dorsal aorta, CP: choroid plexus, DRG: dorsal root ganglion, Fb: forebrain, Ht: heart, Ki: kidney, Li: liver, Lu: lung, NT: neural tube, Sn: snout, TrG: trigeminal ganglion.

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