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Figure 7 | BMC Developmental Biology

Figure 7

From: Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

Figure 7

Localisation of RALDH2 enzyme in day 14.5 male and female embryonic chicken gonads. (A) In males, RALDH2 (arrows; red) localises in Sertoli cells of seminiferous cords. DAPI-stained nuclei delineate germ cells that appear negative for RALDH2 (arrowheads). (B) Double staining with the germ cell marker, GCNA, shows RALDH2 is somatic cells (Sertoli cells; red), but not in many germ cells (arrowheads, green). Some germ cells appear to stain for both proteins (yellow; asterisked arrowheads). (C) RALDH2 expression in somatic (pre-follicular) cells of the left cortex (arrows; red). SOX2+ germ cells (arrowheads; green) are negative for RALDH2. (D) Double-staining of the left ovarian cortex, showing an outer zone of RALDH2+ somatic cells (red; arrows) and underlying germ cells that are GCNA+ (PGCs; arrowheads; green). (E) Double staining of cortical somatic cells reveals GATA4+ nuclei (green) in cells that are also positive for RALDH2 in the cytoplasm (red).

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