Figure 4
From: Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid
STRA8 mRNA expression in embryonic chicken gonads, as assessed by whole mount in situ hybridisation and quantitative real time PCR (qRT-PCR). (A) In females, expression is specific to cortex of the left gonad, and is first detectable by whole mount in situ hybridisation at day 14.5. No STRA8 mRNA expression is detectable in male gonads. Scale bar in whole mounts = 1 mm, scale bar in tissue sections = 100 μm. (B) STRA8 qRT-PCR. STRA8 is up-regulated female-specifically from day 12.5. A low level of expression is detectable in male gonads throughout development. Mean +/- SEM.