Figure 3
From: Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid
Expression of SCP3 mRNA in embryonic chicken gonads. A) Whole mount in situ hybridisation. Strong SCP3 mRNA expression is seen in the left and right female gonads from day 15.5 (arrow and arrowhead). A lower level of expression is detectable in male gonads at the same time (arrowheads). Over-stained and sectioned whole mounts show bilateral expression in the seminiferous cords of developing testes. In females, mRNA expression is localised to the cortex of the left gonad, and in scattered cells of the left and right medulla. Scale bar on whole mounts = 1 mm, scale bar on sections = 100 μm. B) Quantitative real time PCR (qRT-PCR) for SCP3 mRNA expression. A low level of expression is detectable in male gonads throughout development (open circles), while expression increases in female gonads during the time of meiosis (closed circles).